Effects and mechanism of puerarin on the human retinoblastoma cells

J Cell Biochem. 2018 Jun;119(6):4506-4513. doi: 10.1002/jcb.26565. Epub 2018 Feb 28.

Abstract

Puerarin is an isoflavonoid that is extracted from Kudzu root and is considered to have an anti-tumor effect. In the present study, the effects of puerarin on human retinoblastoma (RB) cells and the related pathways was determined. The retinoblastoma RB cell lines were used in this study. Cell viability and colony formation capacity were measured by MTT and colony formation assays. Cell cycle was determined by flow cytometry. Cell migration and invasion were examined by Transwell assay. The expression of cell cycle, EMT, and MAPK/ERK signal pathway-related proteins were detected by western blot following puerarin treatment. The results revealed that cell viability and proliferation of RB cells treated with puerarin were significantly lower in RB cells compared to the control group. Puerarin significantly decreased the proportion of cells during S phase which was accompanied with increase in cells at G0/1 and G2 phases. Moreover, puerarin suppressed cell migration, invasion and up-regulated E-Cadherin expression as well as down-regulated Vimentin and α-SMA expression. Furthermore, puerarin treatment suppressed the expression of p-MEK and p-ERK in RB cells. Our findings suggest that puerarin contributes to in the treatment of RB and other malignant tumors.

Keywords: invasion; migration; proliferation; puerarin; retinoblastoma.

Publication types

  • Retracted Publication

MeSH terms

  • Cell Cycle / drug effects*
  • Cell Line, Tumor
  • Cell Movement / drug effects*
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Isoflavones / pharmacology*
  • Neoplasm Proteins / biosynthesis*
  • Retinoblastoma / drug therapy*
  • Retinoblastoma / metabolism
  • Retinoblastoma / pathology

Substances

  • Isoflavones
  • Neoplasm Proteins
  • puerarin