Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12

Mol Nutr Food Res. 2018 Mar;62(6):e1700616. doi: 10.1002/mnfr.201700616. Epub 2018 Mar 12.


Scope: The aim of this study is to investigate the signaling pathways by which allyl isothiocyanate (AITC) reduces adipocyte differentiation and the efficacy of AITC in suppressing galectin-12 levels as a therapeutic for high fat diet (HFD)-induced obesity.

Methods and results: AITC presents anti-adipogenic effects on 3T3-L1 cells by decreasing lipid droplet accumulation in a dose-dependent manner. AITC suppresses 3T3-L1 differentiation into adipocytes by decreasing galectin-12 expression and by downregulating key adipogenic transcription factors. AITC influences the expression of 3T3-L1 pre-adipocytes by modulating adipokine expression (leptin and resistin) and by regulating the protein kinase B (PKB/Akt)/cAMP response element-binding protein (CREB) pathway. In HFD-fed mice, oral administration of AITC reduces the body weight, accumulation of lipid droplets in the liver, and white adipocyte size.

Conclusion: In summary, the results indicate that AITC inhibits adipocyte differentiation by suppressing galectin-12 levels in 3T3L1 cells and has antiobesity effects in HFD-fed mice.

Keywords: 3T3-L1; Allyl isothiocyanate; adipocytes; galectin-12; obesity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology
  • Adipocytes / drug effects*
  • Adipocytes / metabolism
  • Adipokines / genetics
  • Animals
  • Atherosclerosis / etiology
  • Cell Differentiation / drug effects
  • Cyclic AMP Response Element-Binding Protein / physiology
  • Galectin 2 / antagonists & inhibitors*
  • Isothiocyanates / pharmacology
  • Isothiocyanates / therapeutic use*
  • Lipid Metabolism / drug effects
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity / drug therapy*


  • Adipokines
  • Cyclic AMP Response Element-Binding Protein
  • Galectin 2
  • Isothiocyanates
  • allyl isothiocyanate