Abstract
This report describes the design and evaluation of phosphorylated 7-ethyl-10-hydroxycamptothecin (SN38-P), which selectively eliminates tumor-forming proliferative stem cells, including human induced pluripotent stem cells (hiPSCs) and neural stem cells, from iPSC-derived neural cell mixtures. Results of the present study demonstrate that simple phosphorylation of an anticancer drug can provide a safe, cost-effective, and chemically-defined tool for decontaminating hiPSC-derived neuron.
MeSH terms
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Alkaline Phosphatase / metabolism
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Animals
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Antineoplastic Agents / chemical synthesis
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / metabolism
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Antineoplastic Agents / pharmacology*
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Camptothecin / analogs & derivatives*
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Camptothecin / chemical synthesis
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Camptothecin / chemistry
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Camptothecin / metabolism
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Camptothecin / pharmacology
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Cattle
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Cell Line
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Cell Membrane Permeability
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DNA Damage
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Humans
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Induced Pluripotent Stem Cells / cytology
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Induced Pluripotent Stem Cells / drug effects*
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Induced Pluripotent Stem Cells / enzymology
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Irinotecan
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Neural Stem Cells / cytology
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Neural Stem Cells / drug effects*
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Neural Stem Cells / enzymology
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Neurons / cytology
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Neurons / drug effects
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Phosphorylation
Substances
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Antineoplastic Agents
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Irinotecan
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Alkaline Phosphatase
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Camptothecin