Guidelines for authors and reviewers on antibody use in physiology studies

Am J Physiol Heart Circ Physiol. 2018 Apr 1;314(4):H724-H732. doi: 10.1152/ajpheart.00512.2017. Epub 2018 Jan 5.


Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches. Listen to this article's corresponding podcast at .

Keywords: antibody; immunoblot analysis; immunoblotting; immunohistochemistry; rigor and reproducibility.

Publication types

  • Guideline
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Webcast

MeSH terms

  • Animals
  • Antibodies / immunology*
  • Antibody Specificity
  • Authorship / standards*
  • Biomedical Research / standards*
  • Consensus
  • Data Accuracy
  • Humans
  • Immunoblotting / standards*
  • Immunohistochemistry / standards*
  • Observer Variation
  • Periodicals as Topic / standards*
  • Physiology / standards*
  • Quality Control
  • Reproducibility of Results


  • Antibodies