A consensus linkage map of lentil based on DArT markers from three RIL mapping populations

PLoS One. 2018 Jan 19;13(1):e0191375. doi: 10.1371/journal.pone.0191375. eCollection 2018.

Abstract

Background: Lentil (Lens culinaris ssp. culinaris Medikus) is a diploid (2n = 2x = 14), self-pollinating grain legume with a haploid genome size of about 4 Gbp and is grown throughout the world with current annual production of 4.9 million tonnes.

Materials and methods: A consensus map of lentil (Lens culinaris ssp. culinaris Medikus) was constructed using three different lentils recombinant inbred line (RIL) populations, including "CDC Redberry" x "ILL7502" (LR8), "ILL8006" x "CDC Milestone" (LR11) and "PI320937" x "Eston" (LR39).

Results: The lentil consensus map was composed of 9,793 DArT markers, covered a total of 977.47 cM with an average distance of 0.10 cM between adjacent markers and constructed 7 linkage groups representing 7 chromosomes of the lentil genome. The consensus map had no gap larger than 12.67 cM and only 5 gaps were found to be between 12.67 cM and 6.0 cM (on LG3 and LG4). The localization of the SNP markers on the lentil consensus map were in general consistent with their localization on the three individual genetic linkage maps and the lentil consensus map has longer map length, higher marker density and shorter average distance between the adjacent markers compared to the component linkage maps.

Conclusion: This high-density consensus map could provide insight into the lentil genome. The consensus map could also help to construct a physical map using a Bacterial Artificial Chromosome library and map based cloning studies. Sequence information of DArT may help localization of orientation scaffolds from Next Generation Sequencing data.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping / methods
  • Consensus Sequence
  • DNA, Plant / genetics
  • Genetic Linkage
  • Genome, Plant
  • Lens Plant / genetics*
  • Polymorphism, Single Nucleotide

Substances

  • DNA, Plant

Grant support

The study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK), project no. COST-111O446. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.