Altered chemomechanical coupling causes impaired motility of the kinesin-4 motors KIF27 and KIF7

J Cell Biol. 2018 Apr 2;217(4):1319-1334. doi: 10.1083/jcb.201708179. Epub 2018 Jan 19.

Abstract

Kinesin-4 motors play important roles in cell division, microtubule organization, and signaling. Understanding how motors perform their functions requires an understanding of their mechanochemical and motility properties. We demonstrate that KIF27 can influence microtubule dynamics, suggesting a conserved function in microtubule organization across the kinesin-4 family. However, kinesin-4 motors display dramatically different motility characteristics: KIF4 and KIF21 motors are fast and processive, KIF7 and its Drosophila melanogaster homologue Costal2 (Cos2) are immotile, and KIF27 is slow and processive. Neither KIF7 nor KIF27 can cooperate for fast processive transport when working in teams. The mechanistic basis of immotile KIF7 behavior arises from an inability to release adenosine diphosphate in response to microtubule binding, whereas slow processive KIF27 behavior arises from a slow adenosine triphosphatase rate and a high affinity for both adenosine triphosphate and microtubules. We suggest that evolutionarily selected sequence differences enable immotile KIF7 and Cos2 motors to function not as transporters but as microtubule-based tethers of signaling complexes.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • COS Cells
  • Catalysis
  • Chlorocebus aethiops
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / genetics
  • Evolution, Molecular
  • Hydrolysis
  • Kinesin / genetics
  • Kinesin / metabolism*
  • Kinetics
  • Microscopy, Fluorescence
  • Microtubules / enzymology*
  • Microtubules / genetics
  • Phylogeny
  • Protein Transport
  • Signal Transduction
  • Single Molecule Imaging / methods

Substances

  • Drosophila Proteins
  • cos protein, Drosophila
  • Adenosine Triphosphate
  • Kif7 protein, mouse
  • Kinesin