EVIR: chimeric receptors that enhance dendritic cell cross-dressing with tumor antigens

Nat Methods. 2018 Mar;15(3):183-186. doi: 10.1038/nmeth.4579. Epub 2018 Jan 22.


We describe a lentivirus-encoded chimeric receptor, termed extracellular vesicle (EV)-internalizing receptor (EVIR), which enables the selective uptake of cancer-cell-derived EVs by dendritic cells (DCs). The EVIR enhances DC presentation of EV-associated tumor antigens to CD8+ T cells primarily through MHCI recycling and cross-dressing. EVIRs should facilitate exploring the mechanisms and implications of horizontal transfer of tumor antigens to antigen-presenting cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Neoplasm / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Cells, Cultured
  • Colonic Neoplasms / immunology*
  • Colonic Neoplasms / metabolism
  • Colonic Neoplasms / pathology
  • Dendritic Cells / immunology*
  • Extracellular Vesicles / immunology
  • Extracellular Vesicles / metabolism*
  • Female
  • Histocompatibility Antigens Class II / immunology
  • Mice
  • Mice, Inbred C57BL
  • Receptors, Chimeric Antigen / metabolism*


  • Antigens, Neoplasm
  • Histocompatibility Antigens Class II
  • Receptors, Chimeric Antigen