OMIP-044: 28-color immunophenotyping of the human dendritic cell compartment

Cytometry A. 2018 Apr;93(4):402-405. doi: 10.1002/cyto.a.23331. Epub 2018 Jan 22.


This work describes the first 30-parameter immunophenotyping of the human dendritic cell (DC) compartment using fluorescent-based flow cytometry. The optimized panel allows for simultaneous detection of 21 myeloid-centric markers distinguishing all canonical DC subsets, with parallel enumeration of monocytes, T and B cells as well as NK cells. Thus, this panel will be useful for extensive phenotyping of immune cells from a variety of human samples limited in size.

Keywords: antigen-presenting cells; dendritic cells; high-dimensional cytometry; human PBMCs; human tissue; immunophenotyping; myeloid cells.

MeSH terms

  • B-Lymphocytes / metabolism
  • Biomarkers / metabolism
  • Color
  • Dendritic Cells / metabolism*
  • Flow Cytometry / methods
  • Humans
  • Immunophenotyping / methods
  • Killer Cells, Natural / metabolism
  • Monocytes / metabolism
  • Myeloid Cells / metabolism
  • T-Lymphocytes / metabolism


  • Biomarkers