An integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration

Nat Commun. 2018 Jan 22;9(1):323. doi: 10.1038/s41467-017-02598-7.

Abstract

As a conductive nanomaterial, graphene has huge potentials in nerve function restoration by promoting electrical signal transduction and metabolic activities with unique topological properties. Polydopamine (PDA) and arginylglycylaspartic acid (RGD) can improve cell adhesion in tissue engineering. Here we report an integrated 3D printing and layer-by-layer casting (LBLC) method in multi-layered porous scaffold fabrication. The scaffold is composed of single-layered graphene (SG) or multi-layered graphene (MG) and polycaprolactone (PCL). The electrically conductive 3D graphene scaffold can significantly improve neural expression both in vitro and in vivo. It promotes successful axonal regrowth and remyelination after peripheral nerve injury. These findings implicate that graphene-based nanotechnology have great potentials in peripheral nerve restoration in preclinical and clinical application.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / pharmacology
  • Cell Adhesion / drug effects
  • Electric Conductivity
  • Graphite / chemistry*
  • Graphite / pharmacology
  • Indoles / chemistry*
  • Indoles / pharmacology
  • Male
  • Materials Testing
  • Nanotechnology / methods
  • Nerve Regeneration / drug effects*
  • Nerve Regeneration / physiology
  • Oligopeptides / chemistry*
  • Oligopeptides / pharmacology
  • Peripheral Nerve Injuries / pathology
  • Peripheral Nerve Injuries / physiopathology
  • Peripheral Nerve Injuries / rehabilitation
  • Polyesters / chemistry
  • Polyesters / pharmacology
  • Polymers / chemistry*
  • Polymers / pharmacology
  • Porosity
  • Primary Cell Culture
  • Printing, Three-Dimensional
  • Rats
  • Rats, Sprague-Dawley
  • Schwann Cells / cytology
  • Schwann Cells / drug effects
  • Schwann Cells / physiology
  • Sciatic Nerve / drug effects
  • Sciatic Nerve / injuries
  • Tissue Engineering / methods*
  • Tissue Scaffolds*

Substances

  • Biocompatible Materials
  • Indoles
  • Oligopeptides
  • Polyesters
  • Polymers
  • polydopamine
  • polycaprolactone
  • Graphite
  • arginyl-glycyl-aspartic acid