The embryonic developmental effect of sedaxane on zebrafish (Danio rerio)

Chemosphere. 2018 Apr;197:299-305. doi: 10.1016/j.chemosphere.2018.01.046. Epub 2018 Jan 12.


The succinate dehydrogenase inhibitor (SDHI) fungicides have been extensively used in agriculture, and some of their potential ecological risks to aquatic organisms have been demonstrated recently. Sedaxane (SDX) is a broad spectrum SDHI fungicide. Despite being extensively used in environment, little is known about its potential developmental effect in zebrafish embryo. This study examined the effects of which SDX triggered in zebrafish through embryonic development assessments. Results show that SDX induced mortality, hatch delay and failure in zebrafish embryos, which were concentration dependent. In addition, several developmental abnormalities were observed at 2 mg/L and higher concentrations, including edema, microcephaly, body deformation, and swim bladder not fully inflated. SDX exposure influenced reactive oxygen species, malondialdehyde, peroxidase, glutathione S-transferase, superoxide dismutase and glutathione in live larvae, which indicated that oxidative stress was caused in zebrafish. Furthermore, SDX induced decrease of succinate dehydrogenase activity in zebrafish larvae. These results provide toxicological data of SDX on developing zebrafish embryo, which could be help for further understanding the potential risk on the environment.

Keywords: Developmental toxicity; Oxidative stress; Sedaxane; Zebrafish embryo.

MeSH terms

  • Anilides / toxicity*
  • Animals
  • Embryo, Nonmammalian / drug effects
  • Embryonic Development / drug effects*
  • Fungicides, Industrial / toxicity*
  • Gene Expression Regulation, Developmental / drug effects
  • Glutathione / metabolism
  • Glutathione Transferase / metabolism
  • Larva / drug effects
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects*
  • Pyrazoles / toxicity*
  • Reactive Oxygen Species / metabolism
  • Succinate Dehydrogenase / antagonists & inhibitors*
  • Superoxide Dismutase / metabolism
  • Zebrafish / embryology*
  • Zebrafish / metabolism


  • Anilides
  • Fungicides, Industrial
  • N-(2-(1,1'-bicyclopropyl)-2-ylphenyl)-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide
  • Pyrazoles
  • Reactive Oxygen Species
  • Malondialdehyde
  • Superoxide Dismutase
  • Succinate Dehydrogenase
  • Glutathione Transferase
  • Glutathione