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Randomized Controlled Trial
. Oct-Dec 2017;10(4):237-243.

Effect of Dialyzable Leukocyte Extract on Chronic Cervicitis in Patients With HPV Infection

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Free PMC article
Randomized Controlled Trial

Effect of Dialyzable Leukocyte Extract on Chronic Cervicitis in Patients With HPV Infection

M P Acosta-Rios et al. J Med Life. .
Free PMC article

Abstract

The objective of the study was to assess the clinical, histopathological and immunochemical changes induced by dialyzable leukocyte extract (DLE) treatment in patients with chronic cervicitis associated to HPV infection. Fifty-four female Mexican patients diagnosed with chronic cervicitis, cervical intra-epithelial neoplasia grade 1 (CIN 1) and HPV infection were divided into two groups: patients treated with placebo and patients treated with DLE. Clinical and colposcopy evaluations were performed before and after treatments. Cervix biopsies were obtained to analyze histopathological features and to determine the local immunological changes by immunohistochemistry analyses. Placebo-treated patients showed no significant changes in the evaluated parameters. Interestingly, in DLE-treated patients, clinical manifestations of cervicitis diminished and 89% of them remitted the colposcopic lesions. Histological analyses of biopsies from DLE-treated patients showed a decreasing leukocyte infiltrate. Immunochemical analyses showed an increased expression of TGF-β, while expression of IFN-γ, PCNA, and IL-32 decreased. Our results suggest that DLE can stimulate innate immunity of cervical mucosae, diminishing chronic cervicitis in HPV-infected patients.

Trial registration: Register ISRCTN16429164 Abbreviations: HPV = Human Papilloma Virus; DLE = Dialyzable leukocyte extract.

Keywords: DLE; HPV; chronic cervicitis.

Figures

Fig. 1
Fig. 1
Comparative colposcopy images of the cervix from representative patients before and after placebo or DLE treatments. The images are representative of patients from each group. The arrows indicate the resolution of acetowhite lesion after the treatment with DLE
Fig. 2
Fig. 2
Representative images of histopathological features of cervix tissues. (A) H-E staining of cervix samples from patients before and after treatment with placebo or DLE. The decrease of the inflammatory process in patients treated with DLE was observed. (B) Semiquantitative analyses of infiltrating leukocytes into the stroma. The asterisk marks statistically significant differences (p<0.05). The analyses were performed counting 16 representative epithelial cervical areas from each sample biopsy.
Fig. 3
Fig. 3
Immunohistochemical detection of PCNA. (A) Representative images of cervix biopsies from a patient before and after treatments. The arrows indicate the nuclear PCNA immunolabeling. The decrease of immunolabeling of PCNA in patients treated with DLE was observed (B) Semiquantitative analysis of PCNA detection. The asterisk marks statistically significant differences (p<0.05). The analyses were performed counting 16 representative epithelial cervical areas from each sample biopsy
Fig. 4
Fig. 4
(A) Immunohistochemical detection of TGF-β, IFN γ and IL-32. Representative images of cervix biopsies from a patient before and after treatments. The increase of immunolabeling of TGF-β and the decrease of INF-γ and IL-32 in patients treated with DLE were observed (B) Semiquantitative analyzes of immunohistochemical staining for TGF-β, IFN γ and IL-32. The asterisk marks statistically significant differences (p<0.05). The analyses were performed counting 16 representative epithelial cervical areas from each sample biopsy

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References

    1. Kovacic MB, Katki HA, Kreimer AR, Sherman ME. Epidemiologic analysis of histologic cervical inflammation: relationship to human papillomavirus infections. Hum Pathol. 2008;39(7):1088–1095. - PubMed
    1. Herbert A, Bergeron C, Wiener H, Schenck U, Klinkhamer P, Bulten J, Arbyn M. European guidelines for quality assurance in cervical cancer screening: recommendations for cervical cytology terminology. Cytopathology. 2007;18(4):213–219. - PubMed
    1. Dasari P, Rajathi S, Kumar SV. Colposcopic evaluation of cervix with persistent inflammatory Pap smear: A prospective analytical study. Cytojournal. 2010;7:16–16. - PMC - PubMed
    1. Ojeda MO, van't Veer C, Fernández Ortega CB, Araña Rosainz Mde J, Buurman WA. Dialyzable leukocyte extract differentially regulates the production of TNFalpha, IL-6, and IL-8 in bacterial component-activated leukocytes and endothelial cells. Inflamm Res. 2005;54(2):74–81. - PubMed
    1. García-Hernández U, Robledo-Avila FH, Alvarez-Jiménez VD, Rodríguez-Cortés O, Wong-Baeza I, Serafín-López J, Aguilar-Anguiano LM, Estrada-Parra S, Estrada-García I, Pérez-Tapia SM, Chacón-Salinas R. Dialyzable leukocyte extracts activate TLR-2 on monocytes. Nat Prod Commun. 2014;9(6):853–856. - PubMed

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