Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis

Methods Mol Biol. 2018;1730:59-72. doi: 10.1007/978-1-4939-7592-1_4.

Abstract

Solid-phase extraction coupled with liquid chromatography tandem mass spectrometry provides a robust and sensitive approach for the identification and quantitation of specialized pro-resolving mediators (lipoxins, resolvins, protectins, and maresins), their pathway markers and the classic eicosanoids. Here, we provide a detailed description of the methodologies employed for the extraction of these mediators from biological systems, setup of the instrumentation, sample processing, and then the procedures followed for their identification and quantitation.

Keywords: Eicosanoids; Flux analysis; Lipid mediator metabololipidomics; Liquid chromatography-tandem mass spectrometry; Maresin; Profiling; Protectin; Resolvin.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Liquid / methods*
  • Docosahexaenoic Acids / analysis
  • Eicosapentaenoic Acid / analysis
  • Humans
  • Lipids / analysis*
  • Lipoxins / analysis
  • Metabolomics / methods*
  • Solid Phase Extraction
  • Tandem Mass Spectrometry / methods*

Substances

  • Lipids
  • Lipoxins
  • Docosahexaenoic Acids
  • Eicosapentaenoic Acid