LC-MS/MS Analysis of Bile Acids

Methods Mol Biol. 2018;1730:103-110. doi: 10.1007/978-1-4939-7592-1_8.

Abstract

Besides their role as lipid solubilizers, bile acids (BAs) are increasingly appreciated as bioactive molecules. They bind to G-protein-coupled receptors and nuclear hormone receptors. So they control their own metabolism and act on lipid and energy metabolism. Here we describe a simple, accurate, and fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of BAs in human plasma/serum.

Keywords: Bile acids; Electrospray ionization; Internal standards; Isomer separation; Liquid chromatography; Method; Quantification; Tandem mass spectrometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bile Acids and Salts / blood*
  • Chromatography, Liquid / methods*
  • Humans
  • Isotope Labeling
  • Reproducibility of Results
  • Tandem Mass Spectrometry / methods*

Substances

  • Bile Acids and Salts