Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis

Methods Mol Biol. 2018;1730:213-225. doi: 10.1007/978-1-4939-7592-1_14.

Abstract

Blood is a widely used biofluid in discovery metabolomic research to search for clinical metabolite biomarkers of diseases. Analyzing the entire human blood metabolome is a major analytical challenge, as blood, after being processed into serum or plasma, contains thousands of metabolites with diverse chemical and physical properties as well as a wide range of concentrations. We describe an enabling method based on high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) for in-depth quantification of the metabolomic differences in comparative blood samples with high accuracy and precision.

Keywords: Blood; Chemical isotope labeling; Dansylation; DmPA; LC-MS; Metabolomics.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / blood*
  • Chromatography, Liquid / methods
  • Humans
  • Isotope Labeling / methods*
  • Mass Spectrometry / methods
  • Metabolome
  • Metabolomics / methods*

Substances

  • Biomarkers