A method is proposed for integral assessment of the propagation of microbial cells and viral parti- cles during seasonal thawing of relic ice wedge layers. The results of on-site and laboratory investigation car- ried out in the upper part of permafrost exposure at Mamontova Gora (Yakutiya, Russia) are presented. To increase reliability of the results, suspensions of two microbial species and two coliphage species were intro- duced as biomarkers directly on the surface of thaing ice and in the meltwater flow. Each of the four different model biological objects was shown to possess unique parameters of movement in the meltwater flow and is able to move 132 m in 25-35 min with the water flow.