Mechanism of Inhibition of Translation Termination by Blasticidin S

J Mol Biol. 2018 Mar 2;430(5):591-593. doi: 10.1016/j.jmb.2018.01.007.

Abstract

Understanding the mechanisms of inhibitors of translation termination may inform development of new antibacterials and therapeutics for premature termination diseases. We report the crystal structure of the potent termination inhibitor blasticidin S bound to the ribosomal 70S•release factor 1 (RF1) termination complex. Blasticidin S shifts the catalytic domain 3 of RF1 and restructures the peptidyl transferase center. Universally conserved uridine 2585 in the peptidyl transferase center occludes the catalytic backbone of the GGQ motif of RF1, explaining the structural mechanism of inhibition. Rearrangement of domain 3 relative to the codon-recognition domain 2 provides insight into the dynamics of RF1 implicated in termination accuracy.

Keywords: RF1; antibiotic; blasticidin S; stop-codon recognition; termination suppression.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / metabolism
  • Catalytic Domain / drug effects
  • Codon, Terminator / metabolism
  • Models, Molecular
  • Nucleosides / antagonists & inhibitors
  • Peptide Chain Termination, Translational / drug effects
  • Peptide Termination Factors / drug effects
  • Peptidyl Transferases / metabolism
  • Protein Biosynthesis / drug effects*
  • Protein Conformation
  • Ribosomes / drug effects
  • Ribosomes / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Codon, Terminator
  • Nucleosides
  • Peptide Termination Factors
  • blasticidin S
  • Peptidyl Transferases