High glucose contributes to the proliferation and migration of non-small cell lung cancer cells via GAS5-TRIB3 axis

Cheng-Zhi Ding; Xu-Feng Guo; Guo-Lei Wang; Hong-Tao Wang; Guang-Hui Xu; Yuan-Yuan Liu; Zhen-Jiang Wu; Yu-Hang Chen; Jiao Wang; Wen-Guang Wang

doi:10.1042/BSR20171014

High glucose contributes to the proliferation and migration of non-small cell lung cancer cells via GAS5-TRIB3 axis

Biosci Rep. 2018 Jan 24;38(2):BSR20171014. doi: 10.1042/BSR20171014. Online ahead of print.

Authors

Affiliations

¹ Department of Thoracic Oncology, Henan Provincial Chest Hospital, Zhengzhou, China, Zhengzhou, China.
² Department of Thoracic Surgery, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China, Shanghai, China.
³ Department of Thoracic Surgery, Henan Provincial Chest Hospital, Zhengzhou, China, Zhengzhou, China.
⁴ Division of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China, Zhengzhou, China.
⁵ Department of Thoracic Oncology, Henan Provincial Chest Hospital, Zhengzhou, China, Zhengzhou, 450008, China xbtumor@163.com.

Abstract

Despite the growing number of studies exhibited an association of diabetes mellitus (DM) and lung cancer progression, the concrete mechanism of DM aggravating lung cancer has not been elucidated. This study was to investigate whether and how high glucose (HG) contribute to the proliferation and migration of non-small cell lung cancer (NSCLC) cells in vitro. In the present study, we confirmed that HG promoted the proliferation and migration of NSCLC cells, and also induced an anti-apoptosis effect on NSCLC cells. Moreover, HG inhibited the expression of GAS5 in NSCLC cells but elevated the protein level of TRIB3. GAS5 overexpression promoted the degradation of TRIB3 protein by ubiquitination and inhibited the HG induced-proliferation, anti-apoptosis and migration of NSCLC cells. Importantly, TRIB3 overexpression reversed the effects of GAS5 on the HG-treated NSCLC cells. Taken together, down-regulated GAS5 by HG significantly enhanced the proliferation, anti-apoptosis and migration in NSCLC cells through TRIB3, thus promoting the carcinogenesis of NSCLC.

Keywords: GAS5; NSCLC cells; TRIB3; high glucose; migration; proliferation.