Isolation of circulating plasma cells from blood of patients diagnosed with clonal plasma cell disorders using cell selection microfluidics

Integr Biol (Camb). 2018 Feb 19;10(2):82-91. doi: 10.1039/c7ib00183e.


Blood samples from patients with plasma cell disorders were analysed for the presence of circulating plasma cells (CPCs) using a microfluidic device modified with monoclonal anti-CD138 antibodies. CPCs were immuno-phenotyped using a CD38/CD56/CD45 panel and identified in 78% of patients with monoclonal gammopathy of undetermined significance (MGUS), all patients with smouldering and symptomatic multiple myeloma (MM), and none in the controls. The burden of CPCs was higher in patients with symptomatic MM compared with MGUS and smouldering MM (p < 0.05). FISH analysis revealed the presence of chromosome 13 deletions in CPCs that correlated with bone marrow results. Point mutations in KRAS were identified, including different mutations from sub-clones derived from the same patient. The microfluidic assay represents a highly sensitive method for enumerating CPCs and allows for the cytogenetic and molecular characterization of CPCs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Cell Separation / instrumentation
  • Cell Separation / methods
  • Clone Cells / pathology
  • Equipment Design
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lab-On-A-Chip Devices*
  • Microfluidics / methods
  • Monoclonal Gammopathy of Undetermined Significance / blood*
  • Monoclonal Gammopathy of Undetermined Significance / genetics
  • Monoclonal Gammopathy of Undetermined Significance / immunology
  • Multiple Myeloma / blood*
  • Multiple Myeloma / genetics
  • Multiple Myeloma / immunology
  • Mutation
  • Plasma Cells / immunology
  • Plasma Cells / pathology*
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Smoldering Multiple Myeloma / blood*
  • Smoldering Multiple Myeloma / genetics
  • Smoldering Multiple Myeloma / immunology
  • Syndecan-1 / blood


  • Antibodies, Monoclonal
  • KRAS protein, human
  • SDC1 protein, human
  • Syndecan-1
  • Proto-Oncogene Proteins p21(ras)