Hyperbaric Oxygen therapy effects on bone regeneration in Type 1 diabetes mellitus in rats

Connect Tissue Res. 2018 Nov;59(6):574-580. doi: 10.1080/03008207.2018.1434166. Epub 2018 Feb 9.


Purpose: The aim of this study was evaluate the effect of HBO on diabetic rats.

Materials and methods: Twenty rats were distributed into four groups (n = 5): Control (C); Control + HBO (CH); Diabetes (D) and Diabetes + HBO (DH). Diabetes was induced by streptozotocin, and bone defects were created in both femurs in all animals. HBO therapy began immediately after surgery and was performed daily in the CH and DH groups. After 7 days, the animals were euthanized. The femurs were removed, demineralized, embedded in paraffin, and histologic images were analyzed.

Results: Qualitative histologic analyses showed more advanced stage bone regeneration in control groups (C and CH) compared with diabetic groups (D and DH). Histomorphometric analysis showed significantly increased bone neoformation in CH compared with the other groups (p < 0.001). Diabetic Group (D) showed decreased bone neoformation compared with non-diabetic groups (C and CH) (p < 0.001); however DH did not differ from C Group (p > 0.05). The mast cell population increased in CH compared with the other groups (C, D, and DH) (p < 0.05). The mast cell population did not differ between D and DH Groups.

Conclusions: This study showed that HBO therapy improved early bone regeneration in diabetic rats and increased the mast cell population only in non-diabetic animals. HBO was shown to be important treatment for minimizing deleterious effects of diabetes on bone regeneration.

Keywords: Bone regeneration; Type 1; diabetes mellitus; femur; hyperbaric oxygen therapy; mast cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Regeneration*
  • Diabetes Mellitus, Type 1 / metabolism
  • Diabetes Mellitus, Type 1 / therapy*
  • Femur / injuries*
  • Femur / metabolism*
  • Femur / pathology
  • Hyperbaric Oxygenation*
  • Male
  • Mast Cells / metabolism
  • Mast Cells / pathology
  • Osteogenesis*
  • Rats
  • Rats, Wistar