An allylated firefly luciferin analogue with luciferase specific response in living cells

Yuma Ikeda; Tsuyoshi Saitoh; Kazuki Niwa; Takahiro Nakajima; Nobuo Kitada; Shojiro A Maki; Moritoshi Sato; Daniel Citterio; Shigeru Nishiyama; Koji Suzuki

doi:10.1039/c7cc09720d

An allylated firefly luciferin analogue with luciferase specific response in living cells

Chem Commun (Camb). 2018 Feb 13;54(14):1774-1777. doi: 10.1039/c7cc09720d.

Authors

Yuma Ikeda¹, Tsuyoshi Saitoh, Kazuki Niwa, Takahiro Nakajima, Nobuo Kitada, Shojiro A Maki, Moritoshi Sato, Daniel Citterio, Shigeru Nishiyama, Koji Suzuki

Affiliation

¹ Department of Applied Chemistry Faculty of Science and Technology, Keio University, Hiyoshi 3-14-1, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan. citterio@applc.keio.ac.jp suzuki@applc.keio.ac.jp.

PMID: 29383338
DOI: 10.1039/c7cc09720d

Abstract

An allylated firefly luciferin was successfully synthesized and its bioluminescence properties were evaluated. When applied to cellular imaging in combination with Eluc, which is one of the commercially available luciferases, this analogue displayed a luciferase-specific bioluminescence signal with prolonged emission (>100 min).

MeSH terms

Allyl Compounds / chemistry
Allyl Compounds / metabolism*
Animals
Biocatalysis
COS Cells
Cell Survival
Chlorocebus aethiops
Fireflies
Firefly Luciferin / chemistry
Firefly Luciferin / metabolism*
Luciferases, Firefly / metabolism*
Luminescence
Molecular Structure
Optical Imaging*

Substances

Allyl Compounds
Firefly Luciferin
Luciferases, Firefly