High Dose of A Conjugated Linoleic Acid Mixture Increases Insulin Resistance in Rats Fed Either A Low Fat or A High Fat Diet

Exp Clin Endocrinol Diabetes. 2018 Jun;126(6):379-386. doi: 10.1055/s-0043-118348. Epub 2018 Jan 31.


Obesity and related diseases are becoming more prevalent. Conjugated linoleic acid (CLA) might be a useful coadjutant treatment helping to decrease fat mass. However, the precise impact of CLA is unclear because the decreased body fat mass is followed by an increase in insulin resistance. This study aimed to evaluate some of the consequences of a high dose of CLA in rats fed a normal low fat or a high fat diet for 30 days. Male Wistar rats were separated into 4 groups (each n = 10): Control group receiving 7% fat (soybean oil); CLA group receiving 4% soybean oil and 3% CLA mixture; animal fat (AF) group, receiving 45% fat (lard); and animal fat plus CLA (AF+CLA) group, receiving 42% lard and 3% CLA mixture. The CLA mixture contained 39.32 mole% c9,t11-CLA and 40.50 mole% t10,c12-CLA. After 30 days, both CLA groups (CLA and AF+CLA groups) developed insulin resistance, with an increase in glucose in the fasting state and in an insulin tolerance test. The CLA group had increased liver weight and percentage of saturated fatty acids in liver and adipose tissue. Feeding the high fat diet resulted in increased hepatic triacylglycerol accumulation and this was exacerbated by dietary CLA. It is concluded that a high dose of CLA mixture increases insulin resistance and exacerbates hepatic steatosis when combined with a high fat diet.

Publication types

  • Comparative Study

MeSH terms

  • Adipose Tissue / drug effects
  • Adipose Tissue / metabolism
  • Adiposity / drug effects
  • Animals
  • Body Composition / drug effects
  • Body Weight / drug effects
  • Diet, Fat-Restricted*
  • Diet, High-Fat*
  • Dietary Fats / pharmacology
  • Insulin Resistance*
  • Linoleic Acids, Conjugated / administration & dosage*
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Rats
  • Rats, Wistar


  • Dietary Fats
  • Linoleic Acids, Conjugated