GC-MS olfactometric and LC-DAD-ESI-MS/MS characterization of key odorants and phenolic compounds in black dry-salted olives

J Sci Food Agric. 2018 Aug;98(11):4104-4111. doi: 10.1002/jsfa.8927. Epub 2018 Apr 2.

Abstract

Background: Olives are processed in different ways depending on consumption habits, which vary between countries. Different de-bittering methods affect the aroma and aroma-active compounds of table olives. This study focused on analyzing the aroma and aroma-active compounds of black dry-salted olives using gas chromatography-mass spectrometry-olfactometry (GC-MS-O) techniques.

Results: Thirty-nine volatile compounds which they have a total concentration of 29 459 µg kg-1 , were determined. Aroma extract dilution analysis (AEDA) was used to determine key aroma compounds of table olives. Based on the flavor dilution (FD) factor, the most powerful aroma-active compounds in the sample were methyl-2-methyl butyrate (tropical, sweet; FD: 512) and (Z)-3-hexenol (green, flowery; FD: 256). Phenolic compounds in table olives were also analyzed by LC-DAD-ESI-MS/MS. A total of 20 main phenolic compounds were identified and the highest content of phenolic compound was luteolin-7-glucoside (306 mg kg-1 ), followed by verbascoside (271 mg kg-1 ), oleuropein (231 mg kg-1 ), and hydroxytyrosol (3,4-DHPEA) (221 mg kg-1 ).

Conclusion: Alcohols, carboxylic acids, and lactones were qualitatively and quantitatively the dominant volatiles in black dry-salted olives. Results indicated that esters and alcohols were the major aroma-active compounds. © 2018 Society of Chemical Industry.

Keywords: GC-MS-O; LC-MS-MS; aroma-active compounds; black dry-salted olive; phenolics; volatile.

MeSH terms

  • Flavoring Agents / chemistry
  • Food Handling
  • Fruit / chemistry
  • Gas Chromatography-Mass Spectrometry
  • Odorants / analysis*
  • Olea / chemistry*
  • Olfactometry
  • Phenol / chemistry*
  • Tandem Mass Spectrometry
  • Volatile Organic Compounds / chemistry*

Substances

  • Flavoring Agents
  • Volatile Organic Compounds
  • Phenol