Effects of six common dietary nutrients on murine intestinal organoid growth

PLoS One. 2018 Feb 1;13(2):e0191517. doi: 10.1371/journal.pone.0191517. eCollection 2018.


The intestinal epithelium of the gastrointestinal (GI) tract constantly renews itself to absorb nutrients and provide protection for the body from the outside world. Since the intestinal epithelium is constantly exposed to various chemicals and dietary components, it is critical to determine which constituents promote or inhibit intestinal epithelium health and growth rate. Intestinal organoids, three-dimensional miniature models of the intestines, represent an ex vivo tool to investigate intestinal physiology and growth patterns. In this study, we measured the growth rates of murine intestinal organoids exposed to various concentrations of different dietary constituents. Results indicate that caffeic acid inhibited organoid growth in a concentration-dependent manner, curcumin exhibited variable effectiveness, and vitamin C had no effect on organoid growth.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascorbic Acid / administration & dosage
  • Caffeic Acids / administration & dosage
  • Chlorogenic Acid / administration & dosage
  • Coumaric Acids / administration & dosage
  • Curcumin / administration & dosage
  • Diet*
  • Gastrointestinal Tract / cytology
  • Gastrointestinal Tract / growth & development
  • Gastrointestinal Tract / physiology*
  • Mice
  • Sodium Glutamate / administration & dosage
  • Stem Cells / cytology


  • Caffeic Acids
  • Coumaric Acids
  • Chlorogenic Acid
  • dihydro-3-coumaric acid
  • Curcumin
  • Ascorbic Acid
  • caffeic acid
  • Sodium Glutamate