COL1A1 promotes metastasis in colorectal cancer by regulating the WNT/PCP pathway

Mol Med Rep. 2018 Apr;17(4):5037-5042. doi: 10.3892/mmr.2018.8533. Epub 2018 Feb 1.

Abstract

Colorectal cancer (CRC) is the third leading cause of cancer‑associated mortality, and is a major health problem. Collagen type I α 1 (COL1A1) is a major component of collagen type I. Recently, it was reported to be overexpressed in a variety of tumor tissues and cells. However, the function of COL1A1 in CRC remains unclear. Herein, the present study demonstrated that COL1A1 was upregulated in CRC tissues and the paired lymph node tissues. Transwell assays showed that COL1A1 promoted CRC cell migration in vitro. Moreover, it was revealed that COL1A1 levels were correlated with those of WNT/planar cell polarity (PCP) signaling pathway genes; inhibition of COL1A1 decreased the expression levels of Ras‑related C3 botulinum toxin substrate 1‑GTP, phosphorylated‑c‑Jun N‑terminal kinase, and RhoA‑GTP, all of which are key genes in the WNT/PCP signaling pathway. These results may indicate the mechanisms underlying the oncogenic role of COL1A1 in CRC. In summary, the present data indicated that COL1A1 may serve as an oncoprotein, and that it may be used as a potential therapeutic target in CRC.

Keywords: WNT/PCP pathway; colorectal cancer; bioinformatics analysis; COL1A1; metastasis.

MeSH terms

  • Biomarkers
  • Cell Line, Tumor
  • Collagen Type I / genetics*
  • Collagen Type I / metabolism
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / metabolism*
  • Colorectal Neoplasms / pathology
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic*
  • Gene Knockdown Techniques
  • Humans
  • Immunohistochemistry
  • Neoplasm Metastasis
  • Neoplasm Staging
  • Prognosis
  • Wnt Signaling Pathway*

Substances

  • Biomarkers
  • Collagen Type I
  • collagen type I, alpha 1 chain