Protective Effects of Blueberry Anthocyanins against H 2 O 2-Induced Oxidative Injuries in Human Retinal Pigment Epithelial Cells

J Agric Food Chem. 2018 Feb 21;66(7):1638-1648. doi: 10.1021/acs.jafc.7b06135. Epub 2018 Feb 7.

Abstract

Blueberry anthocyanins are considered protective of eye health because of their recognized antioxidant properties. In this study, blueberry anthocyanin extract (BAE), malvidin (Mv), malvidin-3-glucoside (Mv-3-glc), and malvidin-3-galactoside (Mv-3-gal) all reduced H2O2-induced oxidative stress by decreasing the levels of reactive oxygen species and malondialdehyde and increasing the levels of superoxide dismutase, catalase, and glutathione peroxidase in human retinal pigment epithelial cells. BAE and the anthocyanin standards enhanced cell viability from 63.69 ± 3.36 to 86.57 ± 6.92% (BAE), 115.72 ± 23.41% (Mv), 98.15 ± 9.39% (Mv-3-glc), and 127.97 ± 20.09% (Mv-3-gal) and significantly inhibited cell apoptosis (P < 0.01 for all). Mitogen-activated-protein-kinase pathways, including ERK1/2 and p38, were involved in the bioactivities. In addition, the anthocyanins decreased vascular-endothelial-cell-growth-factor levels and activated Akt-signal pathways. These combined results supported the hypothesis that blueberry anthocyanins could inhibit the induction and progression of age-related macular degeneration (AMD) through antioxidant mechanisms.

Keywords: age-related macular degeneration; antioxidant; blueberry anthocyanins; oxidative stress; retinal pigment epithelial cells.

MeSH terms

  • Anthocyanins / pharmacology*
  • Antioxidants / pharmacology*
  • Blueberry Plants / chemistry*
  • Catalase / metabolism
  • Epithelial Cells / cytology
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Fruit / chemistry
  • Glutathione Peroxidase / metabolism
  • Humans
  • Hydrogen Peroxide / toxicity*
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects*
  • Protective Agents / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Retinal Pigment Epithelium / cytology*
  • Retinal Pigment Epithelium / drug effects
  • Retinal Pigment Epithelium / metabolism
  • Superoxide Dismutase / metabolism

Substances

  • Anthocyanins
  • Antioxidants
  • Protective Agents
  • Reactive Oxygen Species
  • Malondialdehyde
  • Hydrogen Peroxide
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase