Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 172 (4), 881-887.e7

Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer

Affiliations

Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer

Zhen Liu et al. Cell.

Erratum in

Abstract

Generation of genetically uniform non-human primates may help to establish animal models for primate biology and biomedical research. In this study, we have successfully cloned cynomolgus monkeys (Macaca fascicularis) by somatic cell nuclear transfer (SCNT). We found that injection of H3K9me3 demethylase Kdm4d mRNA and treatment with histone deacetylase inhibitor trichostatin A at one-cell stage following SCNT greatly improved blastocyst development and pregnancy rate of transplanted SCNT embryos in surrogate monkeys. For SCNT using fetal monkey fibroblasts, 6 pregnancies were confirmed in 21 surrogates and yielded 2 healthy babies. For SCNT using adult monkey cumulus cells, 22 pregnancies were confirmed in 42 surrogates and yielded 2 babies that were short-lived. In both cases, genetic analyses confirmed that the nuclear DNA and mitochondria DNA of the monkey offspring originated from the nucleus donor cell and the oocyte donor monkey, respectively. Thus, cloning macaque monkeys by SCNT is feasible using fetal fibroblasts.

Keywords: Genetic editing; Human disease models; Macaque monkey cloning; Non-human primates; Somatic cell nuclear transfer; cumulus cells; epigenetic modulators; fetal fibroblasts; primate neurobiology; reprogramming.

Comment in

Similar articles

See all similar articles

Cited by 27 PubMed Central articles

See all "Cited by" articles

Publication types

MeSH terms

Substances

LinkOut - more resources

Feedback