Up-regulated expression of CD86 on circulating intermediate monocytes correlated with disease severity in psoriasis

J Dermatol Sci. 2018 May;90(2):135-143. doi: 10.1016/j.jdermsci.2018.01.005. Epub 2018 Jan 31.


Background: The number of intermediate monocytes (CD14++CD16+) increases in many inflammatory conditions. However, it is not yet known which functional markers expressed by these populations are linked to the pathogenesis of psoriasis.

Objectives: We evaluated the expression of functional markers on circulating intermediate monocytes. Our goal was to correlate specific populations and their markers with the clinical severity of psoriasis.

Methods: A cohort of 43 psoriatic patients was subjected to analysis. The proportion of intermediate monocytes with CD86 expression was evaluated by flow cytometry. Serum beta defensin-2 levels were measured by ELISA. Immunofluorescent staining was performed in order to identify the presence of CD14+CD16+ cells that co-expressed CD86 in affected skin tissues.

Results: Upregulated expression of CD86 on the intermediate subset (but not the number of intermediate monocytes) correlated with clinical severity as measured by PASI scores and serum beta defensin-2 levels. Immunostaining also showed the presence of CD86+CD14+CD16+ cells in the epidermis and dermis of psoriatic plaques, which was associated with increased epidermal proliferation.

Conclusion: These results suggest that the expression of CD86 on circulating intermediate monocytes could be used as an index in clinical practice and provide novel insights into how these cells join a complex immune network under the pathological conditions of psoriasis.

Keywords: CD86; Intermediate monocytes; Psoriasis.

Publication types

  • Observational Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • B7-2 Antigen / immunology
  • B7-2 Antigen / metabolism*
  • Biomarkers / metabolism
  • Cohort Studies
  • Enzyme-Linked Immunosorbent Assay
  • Epidermal Cells
  • Epidermis / pathology*
  • Female
  • Flow Cytometry
  • Humans
  • Male
  • Middle Aged
  • Monocytes / immunology
  • Monocytes / metabolism*
  • Psoriasis / blood
  • Psoriasis / immunology
  • Psoriasis / pathology*
  • Severity of Illness Index
  • Up-Regulation
  • beta-Defensins / blood


  • B7-2 Antigen
  • Biomarkers
  • CD86 protein, human
  • DEFB4A protein, human
  • beta-Defensins