MonoRes: Automatic and Accurate Estimation of Local Resolution for Electron Microscopy Maps

Structure. 2018 Feb 6;26(2):337-344.e4. doi: 10.1016/j.str.2017.12.018. Epub 2018 Jan 27.


Since the beginning of electron microscopy, resolution has been a critical parameter. In this article, we propose a fully automatic, accurate method for determining the local resolution of a 3D map (MonoRes). The foundation of this algorithm is an extension of the concept of analytic signal, termed monogenic signal. The map is filtered at different frequencies and the amplitude of the monogenic signal is calculated, after which a criterion is applied to determine the resolution at each voxel. MonoRes is fully automatic without compulsory user parameters, with great accuracy in all tests, and is computationally more rapid than existing methods in the field. In addition, MonoRes offers the option of local filtering of the original map based on the calculated local resolution.

Keywords: electron microscopy; local resolution; single particle analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Computer Simulation
  • Cryoelectron Microscopy / methods*
  • Image Processing, Computer-Assisted / methods*
  • Models, Molecular*
  • Software