Potentials and pitfalls of inverse fluorescence correlation spectroscopy

Methods. 2018 May 1:140-141:23-31. doi: 10.1016/j.ymeth.2018.01.005. Epub 2018 Feb 2.


Inverse Fluorescence Correlation Spectroscopy (iFCS) is a variant of FCS where unlabeled particles in solution, or domains in membranes, displace their surrounding, signal-generating molecules and thereby generate fluctuations. iFCS has to date been applied to unlabeled as well as labeled particles and protein molecules, using fluorescence as well as Raman scattering as a signal source, in diffraction-limited detection volumes as well as in nano-wells, and on fixed surfaces as well as in lipid bilayers. This review describes these applications and discusses the potentials and pitfalls when using iFCS.

Publication types

  • Review

MeSH terms

  • Cell Membrane / metabolism
  • Cell Membrane / ultrastructure*
  • Diffusion
  • Fluorescence*
  • Fluorescent Dyes / chemistry
  • Intravital Microscopy / instrumentation
  • Intravital Microscopy / methods*
  • Lipid Bilayers / metabolism
  • Spectrometry, Fluorescence / methods*
  • Spectrometry, Fluorescence / trends
  • Spectrum Analysis, Raman / instrumentation
  • Spectrum Analysis, Raman / methods


  • Fluorescent Dyes
  • Lipid Bilayers