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. 2015 Oct;5(5):332-335.
doi: 10.1016/j.jpha.2015.05.002. Epub 2015 May 12.

Rapid screening and distribution of bioactive compounds in different parts of Berberis petiolaris using direct analysis in real time mass spectrometry

Awantika Singh  1   2 , Vikas Bajpai  1   2 , Mukesh Srivastava  3 , Kamal Ram Arya  2   4 , Brijesh Kumar  1   2
Affiliations
Free PMC article

Rapid screening and distribution of bioactive compounds in different parts of Berberis petiolaris using direct analysis in real time mass spectrometry

Awantika Singh et al. J Pharm Anal. 2015 Oct.
Free PMC article

Abstract

Berberis petiolaris Wall. ex G. Don, an unexplored medicinal plant belonging to the family Berberidaceae, is a large deciduous shrub found in Western Himalaya between 1800-3000 m. Chemical profiling of fruit, leaf, root and stem was done by direct analysis in real time mass spectrometry followed by multivariate analysis for discrimination among the plant parts. The bioactive compounds, including magnoflorine, berberine, jatrorrhizine, thalifendine/berberrubine, demethyleneberberine, reticuline, 8-oxoberberine, N-methyltetrahydroberberine, tetrahydropalmatine, tetrahydroberberine and palmatine, were identified by their exact mass measurement and the corresponding molecular formula of each compound. A comparative study of distribution pattern for all these bioactive alkaloids showed qualitative and quantitative variations in different parts of B. petiolaris. Principal component analysis clearly discriminated each part of B. petiolaris plant.

Keywords: Alkaloids; Berberis petiolaris; DART–TOF–MS; Profiling; Statistical analysis.

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Figures

Fig. 1
Fig. 1
Chemical structure of indentified compounds.
Fig. 2
Fig. 2
DART mass spectra of (A) fruit, (B) leaf, (C) root and (D) stem.
Fig. 3
Fig. 3
(A) PCA plot discriminating plant parts of Berberis petiolaris. (B) Score plot discriminating plant parts of Berberis petiolaris.
See this image and copyright information in PMC

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References

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