A Novel, Well-Resolved Direct Laser Bioprinting System for Rapid Cell Encapsulation and Microwell Fabrication

Adv Healthc Mater. 2018 May;7(9):e1701249. doi: 10.1002/adhm.201701249. Epub 2018 Feb 5.

Abstract

A direct laser bioprinting (DLBP) system is introduced in this work. The DLBP system applies visible-laser-induced photo-crosslinking at a wavelength of 405 nm using the photoinitiator VA-086. It is shown that such a system can fabricate vertical structures with fine features (less than 50 µm) and high cell viability (greater than 95%). Experimental characterizations and theoretical simulations are presented, and good agreement is seen between the experiments and theory. The DLBP system is applied to the fabrication of (1) cell-laden hydrogel microgrids, (2) hydrogel microwells, as well as a test of (3) cell encapsulation, and (4) cell seeding. The DLBP system is found to be a promising tool for bioprinting.

Keywords: biofabrication; bioprinting; microwells; poly(ethylene glycol); tissue engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bioprinting / methods*
  • Cells, Immobilized / cytology*
  • Cells, Immobilized / metabolism
  • Humans
  • Hydrogels / chemistry*
  • Lasers*
  • MCF-7 Cells

Substances

  • Hydrogels