Proteomics Analysis of the Adhesion Activity of Lactobacillus acidophilus ATCC 4356 Upon Growth in an Intestine-Like pH Environment

Proteomics. 2018 Mar;18(5-6):e1700308. doi: 10.1002/pmic.201700308.

Abstract

Many health effects of Lactobacillus acidophilus are desirable among these the adhesion ability is vital to enhance the possibility of colonization and stabilization associated with the gut mucosal barrier. In this study, the growth characteristics and the adhesion activity of L. acidophilus in the intestine-like pH environment (pH 7.5) are identified. The number of bacteria adhering to the HT-29 cells is found with a gradual increase trend (pH 5.5-7.5). This also leads to the morphological changes of L. acidophilus after exposure to different pH environments. Furthermore, with the help of the isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis, 207 proteins are detected differentially expressed at pH of 7.5. The use of GO analysis and KEGG analysis indicates three essential pathways related to the cell envelope peptide-glycan biosynthesis, carbohydrate metabolism, and amino acid metabolism are obviously changed. Adhesion related surface protein fmtB and PrtP are upregulated in pH 7.5 group. While the moonlight proteins like pyruvate kinase, which binds specifically to the mucin layer and inhibits the adhesive activity of L. acidophilus, is found downregulated. These results could be useful to understand the adhesion mechanism of L. acidophilus adapting for the gut mucosal barrier in the intestinal environment.

Keywords: Lactobacillus acidophilus; adhesion activity; intestine-like pH environment; proteomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Adhesion*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • HT29 Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Intestines / microbiology
  • Intestines / physiology*
  • Lactobacillus acidophilus / growth & development*
  • Lactobacillus acidophilus / metabolism*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Proteome / analysis*

Substances

  • Bacterial Proteins
  • Membrane Proteins
  • Proteome