Responses of biofilm microorganisms from moving bed biofilm reactor to antibiotics exposure: Protective role of extracellular polymeric substances

Abstract

EPS can affect the migration of antibiotics in biofilm reactors, however the roles of biofilm EPS on the fate of antibiotics and the protective mechanisms to bacterial community remain unknown. We investigated the transport of three representative antibiotics in the biofilm suspension from a moving bed biofilm reactor. Spectral analysis suggested that proteins dominated the interactions between EPS and antibiotics. The adsorbed amounts of antibiotics onto EPS accounted for 14.5%, 88.2% and 13.1% of total concentration for sulfamethizole, tetracycline and norfloxacin, respectively at the biodegradation stage. The respiratory rates and representative enzymatic activities all experienced declines for biofilm without EPS in exposure to antibiotics. Gene sequencing results indicated that the bacterial community in biofilm without EPS was more vulnerable to antibiotics shocks. Our results demonstrated the protective roles of biofilm EPS in resisting antibiotics stresses, which provides important implications for understanding the bioremediation of antibiotics in biofilm systems.

Keywords: Antibiotics; Bacterial community; Biofilm; Extracellular polymeric substances (EPS); Interaction; Transport.