Tracking the origin, development, and differentiation of hematopoietic stem cells

Curr Opin Cell Biol. 2017 Dec;49:108-115. doi: 10.1016/j.ceb.2018.01.002. Epub 2018 Feb 2.

Abstract

Purpose of review: The hierarchical nature of the hematopoietic system provides an ideal model system to illustrate the features of lineage tracing. We have outlined the utility of lineage tracing methods in establishing the origin and development of hematopoietic cells.

Recent findings: Methods such as CRISPR/Cas9, Polylox barcoding, and single-cell RNA-sequencing have improved our understanding of hematopoiesis.

Summary: This review chronicles the fate of the hematopoietic cells emerging from the mesoderm that subsequently develops into the adult blood lineages. Specifically, we explain classic techniques utilized in lineage tracing for the hematopoietic system, as well as novel state-of-the-art methods to elucidate clonal hematopoiesis and cell fate mapping at a single-cell level.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Differentiation
  • Hematopoiesis / genetics*
  • Hematopoietic Stem Cells / metabolism*
  • Humans