Long Non-coding RNA BC168687 is Involved in TRPV1-mediated Diabetic Neuropathic Pain in Rats

Neuroscience. 2018 Mar 15:374:214-222. doi: 10.1016/j.neuroscience.2018.01.049. Epub 2018 Feb 5.


Long noncoding RNAs (lncRNAs) participate in a diverse range of molecular and biological processes, and dysregulation of lncRNAs has been observed in the pathogenesis of various human diseases. We observed alterations in mechanical withdrawal thresholds (MWT) and thermal withdrawal latencies (TWL) in streptozotocin (STZ)-induced diabetic rats treated with small interfering RNA (siRNA) of lncRNA BC168687. We detected expression of transient receptor potential vanilloid type 1 (TRPV1) in rat dorsal root ganglia (DRG) by a series of molecular experiments. We determined relative levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in rat serum by enzyme-linked immunosorbent assay (ELISA). In addition, we examined extracellular regulated protein kinases (ERK) and p38 mitogen-activated protein kinase (MAPK) signaling pathways by Western blot (WB). We showed that the MWT and TWL of diabetic rats increased significantly compared with control. Expression of TRPV1 receptors in DRG substantially decreased. Relative levels of TNF-α and IL-1β in the serum of lncRNA BC168687 siRNA-treated rats were reduced. Phosphorylation (p)-ERK and p-p38 signaling pathways in DRG were also decreased. Taken together, we concluded lncRNA BC168687 siRNA may alleviate TRPV1-mediated diabetic neuropathic pain.

Keywords: DRG; TRPV1; diabetic neuropathic pain; long non-coding RNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diabetes Mellitus, Experimental / metabolism*
  • Diabetes Mellitus, Experimental / pathology
  • Diabetes Mellitus, Experimental / therapy
  • Diabetic Neuropathies / metabolism*
  • Diabetic Neuropathies / pathology
  • Diabetic Neuropathies / therapy
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Ganglia, Spinal / metabolism
  • Ganglia, Spinal / pathology
  • Interleukin-1beta / blood
  • MAP Kinase Signaling System / physiology
  • Male
  • Neuralgia / metabolism*
  • Neuralgia / pathology
  • Neuralgia / therapy
  • Pain Threshold / physiology
  • RNA, Long Noncoding / antagonists & inhibitors
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • RNA, Small Interfering
  • Rats, Sprague-Dawley
  • Receptors, Purinergic P2X7 / metabolism
  • TRPV Cation Channels / metabolism*
  • Tumor Necrosis Factor-alpha / blood
  • p38 Mitogen-Activated Protein Kinases / metabolism


  • BC168687 long noncoding RNA, rat
  • IL1B protein, rat
  • Interleukin-1beta
  • RNA, Long Noncoding
  • RNA, Small Interfering
  • Receptors, Purinergic P2X7
  • TRPV Cation Channels
  • Trpv1 protein, rat
  • Tumor Necrosis Factor-alpha
  • Extracellular Signal-Regulated MAP Kinases
  • p38 Mitogen-Activated Protein Kinases