Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast

Nat Commun. 2018 Feb 8;9(1):587. doi: 10.1038/s41467-017-02284-8.


Telomerase reverse transcriptase (TERT) and the non-coding telomerase RNA subunit (TR) constitute the core of telomerase. Here we now report that the putative F-box protein Pof8 is also a constitutive component of active telomerase in fission yeast. Pof8 functions in a hierarchical assembly pathway by promoting the binding of the Lsm2-8 complex to telomerase RNA, which in turn promotes binding of the catalytic subunit. Loss of Pof8 reduces TER1 stability, causes a severe assembly defect, and results in critically short telomeres. Structure profile searches identified similarities between Pof8 and telomerase subunits from ciliated protozoa, making Pof8 next to TERT the most widely conserved telomerase subunits identified to date.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Molecular Sequence Data
  • Protein Binding
  • RNA / genetics
  • RNA / metabolism
  • RNA Recognition Motif Proteins / chemistry
  • RNA Recognition Motif Proteins / metabolism*
  • RNA, Fungal / genetics
  • RNA, Fungal / metabolism
  • Schizosaccharomyces / chemistry
  • Schizosaccharomyces / enzymology
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces pombe Proteins / chemistry
  • Schizosaccharomyces pombe Proteins / metabolism*
  • Sequence Alignment
  • Telomerase / chemistry
  • Telomerase / genetics
  • Telomerase / metabolism*
  • Telomere / genetics
  • Telomere / metabolism


  • RNA Recognition Motif Proteins
  • RNA, Fungal
  • Schizosaccharomyces pombe Proteins
  • pof8 protein, S pombe
  • telomerase RNA
  • RNA
  • Telomerase