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. 2018 Feb;34(1):59-64.
doi: 10.5423/PPJ.NT.08.2017.0190. Epub 2018 Feb 1.

First Isolation and Molecular Characterization of Bacteriophages Infecting Acidovorax citrulli, the Causal Agent of Bacterial Fruit Blotch

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Free PMC article

First Isolation and Molecular Characterization of Bacteriophages Infecting Acidovorax citrulli, the Causal Agent of Bacterial Fruit Blotch

Aryan Rahimi-Midani et al. Plant Pathol J. .
Free PMC article

Abstract

Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, and exhibits the morphology of phages in the Myoviridae family with a head diameter of 100 ± 5 nm and tail length of 150 ± 5 nm. ACP17 has eclipse and latent periods of 25 ± 5 min and 50 ± 5 min, respectively, and a burst size of 120. The genome of ACP17 is 156,281 base pairs with a G + C content of 58.7%, 263 open reading frames, and 4 transfer RNA genes. Blast search and phylogenetic analysis of the major capsid protein showed that ACP17 has limited homology to two Stentrophomonas phages, suggesting that ACP17 is a new type of Myoviridae isolated from A. citrulli.

Keywords: BFB; phage; watermelon.

Figures

Fig. 1
Fig. 1
Electron micrograph of ACP17 using negative staining.
Fig. 2
Fig. 2
Stability of ACP17 under various pH (top) and temperature (bottom) conditions analyzed by plaque assay. Results are the average of three replicates and the vertical bars indicate standard deviation.
Fig. 3
Fig. 3
Phylogenic tree based on the amino acid sequences of the major capsid proteins of selected members of Myoviridae and phage ACP17. Bar indicates the number of nucleotide substitutions per site.
Fig. 4
Fig. 4
Comparative analysis of phage open reading frames (ORFs) encoded by phage ACP17 and Stenotrophomonas phage IME-SM1 using using the Easyfig 9 program (http://mjsull.github.io/Easyfig/). Arrows indicate the ORFs and their orientation and lines indicate ORFs with amino acid sequence homology.

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