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. 2018;1706:293-302.
doi: 10.1007/978-1-4939-7471-9_16.

RNA Interference to Knock Down Gene Expression

Free PMC article

RNA Interference to Knock Down Gene Expression

Haiyong Han. Methods Mol Biol. .
Free PMC article


RNA interference (RNAi) is a biological process by which double-stranded RNA (dsRNA) induces sequence-specific gene silencing by targeting mRNA for degradation. As a tool for knocking down the expression of individual genes posttranscriptionally, RNAi has been widely used to study the cellular function of genes. In this chapter, I describe procedures for using gene-specific, synthetic, short interfering RNA (siRNA) to induce gene silencing in mammalian cells. Protocols for using lipid-based transfection reagents and electroporation techniques are provided. Potential challenges and problems associated with the siRNA technology are also discussed.

Keywords: Electroporation; Gene silencing; RNA interference; RNAi; Transfection; siRNA.

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