The No-Nonsens SDS-PAGE

Lars Backman; Karina Persson

doi:10.1007/978-1-4939-7546-4_8

The No-Nonsens SDS-PAGE

Methods Mol Biol. 2018:1721:89-94. doi: 10.1007/978-1-4939-7546-4_8.

Authors

Lars Backman¹, Karina Persson²

Affiliations

¹ Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden. lars.backman@chem.umu.se.
² Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden.

PMID: 29423849
DOI: 10.1007/978-1-4939-7546-4_8

Abstract

The discontinuous polyacrylamide gel electrophoresis system devised by Laemmli (Nature 227:680-685, 1970) has not only been used in numerous laboratories but has also been modified in several ways since its birth. In our laboratories, we use a modified Laemmli SDS-PAGE system for following protein purification as well as for analysis of certain protein-protein interactions, mainly involving filametous actin.

Keywords: SDS-PAGE; polyacrylamide gel electrophoresis.

Publication types

Review

MeSH terms

Electrophoresis, Polyacrylamide Gel / methods*