Proteins act as executors for almost all kinds of cellular processes. The majority of proteins achieve their proper functions through interacting with other proteins. Knowing the binding partners of a protein is instrumental for understanding its function. The antibody pull-down method is a powerful and common approach to detect protein-protein interactions. Here, an antibody pull-down protocol is described for detecting protein-protein interactions in fission yeast Schizosaccharomyces pombe.
Keywords: Antibody; Co-immunoprecipitation; Fission yeast; In vivo; Protein-protein interaction; Pull-down; Schizosaccharomyces pombe.