Protective and anti-inflammatory effects of hydroalcoholic leaf extract of Origanum vulgare on oxidative stress, TNF-α gene expression and liver histological changes in paraquat-induced hepatotoxicity in rats

Arch Physiol Biochem. 2019 Feb;125(1):56-63. doi: 10.1080/13813455.2018.1437186. Epub 2018 Feb 9.

Abstract

Context: Toxicity with paraquat can lead to serious damages to the liver.

Objective: The study investigates the protective effects of Origanum vulgare leaf extract against paraquat liver damage.

Material and methods: Rats were divided into six groups. Group 1, the control group; group 2, rats that received paraquat only; group 3, rats that received paraquat plus silymarin; and groups 4, 5, and 6 were treated with paraquat and O. vulgare leaf extract. Then, the serum and tissue parameters of the oxidative stress were examined.

Results: In group 2, paraquat caused a remarkable increase in the level of serum ALT, AST, ALP, lipid profiles, and liver TNF-α gene expression compared to group 1. The groups which received O. vulgare leaf extract exhibited significant ameliorations in abnormalities of paraquat-induced liver damage and serum biochemical parameters.

Conclusion: O. vulgare leaf extract has inhibitory effects on paraquat-induced liver damage due to its antioxidant properties.

Keywords: Hepatotoxicity; histological changes; oxidative stress; paraquat.

MeSH terms

  • Alcohols / chemistry
  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Antioxidants / pharmacology
  • Blood Proteins / metabolism
  • Catalase / metabolism
  • Cytoprotection / drug effects
  • Gene Expression Regulation / drug effects
  • Liver / cytology
  • Liver / drug effects*
  • Liver / metabolism
  • Male
  • Malondialdehyde / blood
  • Origanum / chemistry*
  • Oxidative Stress / drug effects*
  • Paraquat / toxicity*
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry*
  • Protein Carbonylation / drug effects
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Tumor Necrosis Factor-alpha / blood
  • Tumor Necrosis Factor-alpha / metabolism*

Substances

  • Alcohols
  • Anti-Inflammatory Agents
  • Antioxidants
  • Blood Proteins
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Malondialdehyde
  • Catalase
  • Superoxide Dismutase
  • Paraquat