Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance

Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):3174-3179. doi: 10.1073/pnas.1705927115. Epub 2018 Feb 5.


Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci have been reported. Here, we report the identification and characterization of the rice bsr-k1 (broad-spectrum resistance Kitaake-1) mutant, which confers broad-spectrum resistance against Magnaporthe oryzae and Xanthomonas oryzae pv oryzae with no major penalty on key agronomic traits. Map-based cloning reveals that Bsr-k1 encodes a tetratricopeptide repeats (TPRs)-containing protein, which binds to mRNAs of multiple OsPAL (OsPAL1-7) genes and promotes their turnover. Loss of function of the Bsr-k1 gene leads to accumulation of OsPAL1-7 mRNAs in the bsr-k1 mutant. Furthermore, overexpression of OsPAL1 in wild-type rice TP309 confers resistance to M. oryzae, supporting the role of OsPAL1 Our discovery of the bsr-k1 allele constitutes a significant conceptual advancement and provides a valuable tool for breeding broad-spectrum resistant rice.

Keywords: TPR protein; blast disease; broad-spectrum resistance; innate immunity; rice.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cytoplasm / metabolism
  • Disease Resistance / genetics
  • Gene Expression Regulation, Plant
  • Magnaporthe / pathogenicity
  • Mutation
  • Oryza / genetics
  • Oryza / microbiology
  • Oryza / physiology*
  • Plant Diseases / genetics*
  • Plant Diseases / microbiology
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plants, Genetically Modified / genetics
  • Protein Domains
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism
  • Repetitive Sequences, Amino Acid
  • Xanthomonas / pathogenicity


  • Plant Proteins
  • RNA, Messenger
  • RNA-Binding Proteins