Changes in polyphenol composition and bioactivity of the native Chilean white strawberry (Fragaria chiloensis spp. chiloensis f. chiloensis) after in vitro gastrointestinal digestion

Food Res Int. 2018 Mar;105:10-18. doi: 10.1016/j.foodres.2017.10.074. Epub 2017 Nov 8.

Abstract

The Chilean white strawberry (Fragaria chiloensis spp. chiloensis f. chiloensis) is a semi-domesticated strawberry with high polyphenol content and antioxidant activity occurring in southern Chile. The aim of this work was to compare the composition and bioactivity of the polyphenol-enriched fruit extract (PEE) before and after simulated gastrointestinal digestion (GID). Results show a decrease by >50% in the total phenolic (TP) content at the end of the GID, compared to the non-digested PEE. A reduction in the antioxidant capacity of the PEEs was observed after GID by means of DPPH, FRAP, TEAC and anion superoxide assays. After simulated GID the PEE significantly inhibited α-glucosidase with an IC50 value of 3.13μg/mL. The inhibition of pancreatic lipase was reduced by 95% after GID. All the PEEs did not show inhibitory effect towards α-amylase throughout the GID. In the same way, the PEEs did not significantly protect human gastric adenocarcinoma (AGS) cells against H2O2-induced stress. Thirty eight compounds were tentatively identified in the non-digested PEE. The compounds that were more affected by the simulated GID were simple phenolics. After the GID, only 33 and 25 compounds were detected, in the gastric and intestinal steps, respectively. These results evidence the changes elicited by GID on the bioactivity and polyphenolic composition of the white strawberry.

Keywords: Antioxidant activity; Fragaria chiloensis; HPLC-DAD-MS/MS(n); In vitro digestion; Metabolic syndrome-associated enzymes; Polyphenolic profiles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry
  • Cell Line
  • Chile
  • Digestion*
  • Epithelial Cells / cytology
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Fragaria / chemistry*
  • Fruit / chemistry
  • Gastrointestinal Tract / cytology
  • Gastrointestinal Tract / drug effects
  • Gastrointestinal Tract / metabolism*
  • Humans
  • Hydrogen Peroxide / metabolism
  • Inhibitory Concentration 50
  • Oxidative Stress / drug effects
  • Plant Extracts / chemistry
  • Polyphenols / chemistry*
  • alpha-Amylases / antagonists & inhibitors
  • alpha-Amylases / metabolism
  • alpha-Glucosidases / metabolism

Substances

  • Antioxidants
  • Plant Extracts
  • Polyphenols
  • Hydrogen Peroxide
  • alpha-Amylases
  • alpha-Glucosidases