The Effects of different nitrogen doses on antioxidant and antimicrobial activity of Stevia (Stevia rebaudiana Bert.)

Cell Mol Biol (Noisy-le-grand). 2018 Feb 10;64(2):39-45. doi: 10.14715/cmb/2018.64.2.8.

Abstract

In this study, the efficiency of the different nitrogen doses (0, 5, 10, 15 and 20 kg ha-1) on biological activity levels (antioxidant and antimicrobial activity) of Stevia rebaudiana Bert. was investigated. In addition, methanol extracts were obtained by maceration method from different doses of fertilizer applied stevia. The components in methanol extracts of plants were determined by gas chromatography-mass spectrometry (GC-MS) method. Antimicrobial activities of stevia extracts were investigated by microdilution method. The antioxidant activity evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric thiocyanate (FTC), thiobarbituric acid (TBA), reducing power, total phenol content (TPC), and total flavonoid content (TFC) methods. According to the results, the fertilizer doses effects on antimicrobial activity of stevia were not made much difference. But in antioxidant activity, there were some variations in the activity-dependent on fertilizer amount.

Keywords: Antimicrobial; Antioxidant; Essential oil; Stevia rebaudiana..

MeSH terms

  • Anti-Infective Agents / chemistry
  • Anti-Infective Agents / isolation & purification
  • Anti-Infective Agents / pharmacology*
  • Antioxidants / chemistry*
  • Benzene Derivatives / analysis
  • Biphenyl Compounds / chemistry
  • Dose-Response Relationship, Drug
  • Fertilizers
  • Flavonoids / analysis
  • Iron / chemistry
  • Methanol / chemistry
  • Nitrates / pharmacology*
  • Picrates / chemistry
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry
  • Plant Leaves / drug effects
  • Stevia / chemistry
  • Stevia / drug effects*
  • Thiobarbiturates / chemistry
  • Thiocyanates / chemistry

Substances

  • Anti-Infective Agents
  • Antioxidants
  • Benzene Derivatives
  • Biphenyl Compounds
  • Fertilizers
  • Flavonoids
  • Nitrates
  • Picrates
  • Plant Extracts
  • Thiobarbiturates
  • Thiocyanates
  • ferric thiocyanate
  • 1,1-diphenyl-2-picrylhydrazyl
  • Iron
  • thiobarbituric acid
  • Methanol