Mining Exosomal MicroRNAs from Human-Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration

Methods Mol Biol. 2018:1733:127-136. doi: 10.1007/978-1-4939-7601-0_10.


Myocardial infarction is the leading cause of morbidity and mortality worldwide. Recent advances in cardiac regenerative therapy have allowed for novel modalities in replenishing the damaged myocardium. However, poor long-term engraftment and survival of transplanted cells have largely precluded effective cell replacement. As an alternative to direct cell replacement, the release of paracrine protective factors may be a more plausible effector for cardioprotection which may partially be mediated through secretion of microvesicles, or exosomes, that contribute to cell-cell communication. In this chapter, we describe the isolation of exosomes from induced pluripotent stem cells-derived cardiomyocytes for subsequent microRNA profiling for a better understanding of the biological cargo contained within exosomes.

Keywords: Exosomes; Heart; Stem cell; iPSC-CMs; microRNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Differentiation / genetics
  • Culture Media, Conditioned / pharmacology
  • Data Mining
  • Exosomes / metabolism*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • MicroRNAs / genetics*
  • Myocardium / metabolism*
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / metabolism*
  • Regeneration*


  • Culture Media, Conditioned
  • MicroRNAs