Long non‑coding RNA BC168687 small interfering RNA reduces high glucose and high free fatty acid‑induced expression of P2X7 receptors in satellite glial cells

Mol Med Rep. 2018 Apr;17(4):5851-5859. doi: 10.3892/mmr.2018.8601. Epub 2018 Feb 13.


Purinergic signaling contributes to inflammatory and immune responses. The activation of the P2X purinoceptor 7 (P2X7) in satellite glial cells (SGCs) may be an essential component in the promotion of inflammation and neuropathic pain. Long non‑coding RNAs (lncRNAs) are involved in multiple physiological and pathological processes. The aim of the present study was to investigate the effects of a small interfering RNA for the lncRNA BC168687 on SGC P2X7 expression in a high glucose and high free fatty acids (HGHF) environment. It was demonstrated that BC168687 small interfering (si)RNA downregulated the co‑expression of the P2X7 and glial fibrillary acidic protein and P2X7 mRNA expression. Additionally, HGHF may activate the mitogen‑activated protein kinase signaling pathway by increasing the release of nitric oxide and reactive oxygen species in SGCs. Taken together, these results indicate that silencing BC168687 expression may downregulate the increased expression of P2X7 receptors in SGCs induced by a HGHF environment.

Keywords: long non-coding RNA; BC168687; high glucose and high free fatty acids; P2X purinoceptor 7; glial fibrillary acidic protein.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Blood Glucose
  • Cell Survival
  • Cellular Microenvironment
  • Fatty Acids, Nonesterified / metabolism*
  • Gene Expression Regulation*
  • Gene Silencing
  • Glial Fibrillary Acidic Protein / genetics
  • Glial Fibrillary Acidic Protein / metabolism
  • MAP Kinase Signaling System
  • Male
  • Neuroglia / metabolism*
  • Nitric Oxide / metabolism
  • RNA Interference*
  • RNA, Long Noncoding / genetics*
  • RNA, Messenger / genetics
  • RNA, Small Interfering / genetics*
  • Rats
  • Reactive Oxygen Species / metabolism
  • Receptors, Purinergic P2X7 / genetics*


  • BC168687 long noncoding RNA, rat
  • Blood Glucose
  • Fatty Acids, Nonesterified
  • GFAP protein, rat
  • Glial Fibrillary Acidic Protein
  • RNA, Long Noncoding
  • RNA, Messenger
  • RNA, Small Interfering
  • Reactive Oxygen Species
  • Receptors, Purinergic P2X7
  • Nitric Oxide
  • Adenosine Triphosphate