pSTM6-275, a Conjugative IncHI2 Plasmid of Salmonella enterica That Confers Antibiotic and Heavy-Metal Resistance under Changing Physiological Conditions

Antimicrob Agents Chemother. 2018 Apr 26;62(5):e02357-17. doi: 10.1128/AAC.02357-17. Print 2018 May.

Abstract

Detailed annotation of an IncHI2 plasmid, pSTM6-275, from Salmonella enterica serotype 1,4,5,12:i:- strain TW-Stm6 revealed a composite structure, including antimicrobial resistance genes on mobile genetic elements. The plasmid was thermosensitive for transfer to Escherichia coli and conferred reduced susceptibility to antibiotics, copper sulfate, and silver nitrate. Metal ion susceptibility was dependent on physiological conditions, giving an insight into the environments where this trait might confer a fitness advantage.

Keywords: IncHI2; Salmonella; copper; heavy metals; integrons; plasmid-mediated resistance; silver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Drug Resistance, Multiple, Bacterial / genetics
  • Integrons / genetics
  • Metals, Heavy / pharmacology*
  • Microbial Sensitivity Tests
  • Plasmids / genetics*
  • Salmonella enterica / drug effects*
  • Salmonella enterica / genetics*

Substances

  • Anti-Bacterial Agents
  • Metals, Heavy