Data from the representative 2013 Eurobarometer survey were combined with macro-environmental data to assess relationships with different domains of physical activity (PA) in 28 European Union countries. Higher mean annual temperatures were the only macro-environmental factor found to be associated with levels of physical activity; an increase in the mean annual temperature by 1°C was associated with-0.94 fewer minutes of vigorous-intensity activity per week (95% CI: -1.66 to -0.23). This highlights the importance of modifiable influences (e.g. opportunities for active travel) on PA and underscores the potential of public health interventions to raise levels of physical activity.