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. 2018 Feb 9;13:895-901.
doi: 10.2147/IJN.S148390. eCollection 2018.

Increased Viability of Fibroblasts When Pretreated With Ceria Nanoparticles During Serum Deprivation

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Free PMC article

Increased Viability of Fibroblasts When Pretreated With Ceria Nanoparticles During Serum Deprivation

Francielli S Genier et al. Int J Nanomedicine. .
Free PMC article

Abstract

Conditions of cellular stress are often the cause of cell death or dysfunction. Sustained cell stress can lead to several health complications, such as extensive inflammatory responses, tumor growth, and necrosis. To prevent disease and protect human tissue during these conditions and to avoid medication side effects, nanomaterials with unique characteristics have been applied to biological systems. This paper introduces the pretreatment in human dermal fibroblasts with cerium oxide nanoparticles during nutritional stress. For this purpose, human dermal fibroblast cells received cell culture media with concentrations of 250 µg/mL and 500 µg/mL of nano-cerium oxide before being exposed to 24, 48, and 72 hours of serum starvation. Contrast images demonstrated higher cell confluence and cell integrity in cells pretreated with ceria nanoparticles compared to untreated cells. It was confirmed by MTS assay after 72 hours of serum starvation that higher cell viability was achieved with ceria nanoparticles. The results demonstrate the potential of cerium oxide nanoparticles as protective agents during cellular starvation.

Keywords: cerium oxide; human dermal fibroblasts; nanoparticles; serum starvation.

Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Experiment paradigm. Abbreviations: FBS, fetal bovine serum; HDF, human dermal fibroblast; NP, nanoparticle.
Figure 2
Figure 2
TEM image of CeriaNPs. Abbreviations: NP, nanoparticle; Mag, magnification; TEM, transmission electron microscopy.
Figure 3
Figure 3
HDF cells exposed to ceria NP (250 and 500 µg/mL for 24, 48, and 72 hours). Notes: After-preincubation phase contrast images were collected, 10% FBS concentration (control) after 24 hours (A), 48 hours (B), and 72 hours (C). First column shows images with no pretreatment, while the second and third columns show images of the wells pretreated with 250 µg/mL and 500 µg/mL of ceria NP, respectively. Magnification: 100×. Cells were recovered in fresh medium for 24 hours. Abbreviations: FBS, fetal bovine serum; HDF, human dermal fibroblasts; NP, nanoparticle.
Figure 4
Figure 4
NP-pretreated HDF cells were stressed by reducing the FBS concentration to 0.2%. Notes: Phase contrast images were collected, after 24 hours (A), 48 hours (B), and 72 hours (C). The first column shows images with no pretreatment, while the second and third columns show images of wells pretreated with 250 µg/mL and 500 µg/mL of ceria NP, respectively. Magnification: 100×. Cells were recovered in fresh medium for 24 hours. Abbreviations: FBS, fetal bovine serum; HDF, human dermal fibroblasts; NP, nanoparticle.
Figure 5
Figure 5
NP-pretreated HDF cells were stressed by reducing the FBS concentration to 0.1%. Notes: Phase contrast images were collected, after 24 hours (A), 48 hours (B), and 72 hours (C). First column shows images with no pretreatment, while second and third columns show images of wells pretreated with 250 µg/mL and 500 µg/mL of ceria NP, respectively. Magnification: 100×. Cells were recovered in fresh medium for 24 hours. Abbreviations: FBS, fetal bovine serum; HDF, human dermal fibroblasts; NP, nanoparticle.
Figure 6
Figure 6
Quantitative analysis of cell viability by MTS. Notes: Viability measurements of HDF cells under normal 10% FBS and different stressed conditions, 0.2% FBS and 0.1% FBS, without ceria NP, and cells exposed to ceria NP at 250 µg/mL and 500 µg/mL. The values were normalized in order to present the control (no Ceria NP with 10% FBS) as 100% in cell viability. Data = mean ± SEM; N=3; at respective concentrations: *P<0.01 compared to next lowest FBS percentage, same NP treatment, and same time period; **P<0.05 compared to respective control at the same FBS percentage. Abbreviations: FBS, fetal bovine serum; HDF, human dermal fibroblasts; NP, nanoparticle; SEM, standard error of the mean.

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References

    1. Shcherbakov AB, Zholobak NM, Baranchikov AE, Ryabova AV, Ivanov VK. Cerium fluoride nanoparticles protect cells against oxidative stress. Mat Sci Eng C. 2015;50:151–159. - PubMed
    1. Castano CE, O’Keefe MJ, Fahrenholtz WG. Cerium-based oxide coatings. Curr Opin Solid State Mater Sci. 2015;19(2):69–76.
    1. Xia T, Kovochich M, Liong M, et al. Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano. 2008;2(12):2121–2134. - PMC - PubMed
    1. Montfort C, Alili L, Teuber-Hanselmann S, Brenneisen P. Redox-active cerium oxide nanoparticles protect human dermal fibroblasts from PQ-induced damage. Redox Biol. 2015;4:1–5. - PMC - PubMed
    1. Weaver JD, Stabler CL. Antioxidant cerium oxide nanoparticle hydrogels for cellular encapsulation. Acta Biomater. 2015;16:136–144. - PMC - PubMed
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