Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells

Saudi J Gastroenterol. Jan-Feb 2018;24(1):59-66. doi: 10.4103/sjg.SJG_333_17.

Abstract

Background/aims: Probiotics play a role in relieving irritable bowel syndrome (IBS); however, the underlying mechanism is yet unclear. The aim of the study was to investigate the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein.

Materials and methods: HT-29 and Caco-2 cells were treated with different concentrations of L. acidophilus and B. longum supernatants for 12 h and 24 h, respectively. SERT mRNA and proteins levels were detected by real-time polymerase chain reaction (real-time PCR) and Western-blotting.

Results: The mRNA levels of SERT in HT-29 and Caco-2 cells treated with different concentrations of L. acidophilus or B. longum supernatants for 12 h and 24 h, each, were higher than that in the control groups. In addition, the expression of the protein in both cells was also upregulated, which was approximately similar to that of the corresponding mRNA.

Conclusions: L. acidophilus and B. longum supernatants can upregulate SERT mRNA and protein levels in intestinal epithelial cells.

Keywords: Bifidobacterium longum supernatant; Lactobacillus acidophilus supernatant; intestinal epithelial cells; serotonin transporter.

MeSH terms

  • Bifidobacterium longum / metabolism*
  • Caco-2 Cells
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism
  • Gene Expression Regulation / drug effects
  • HT29 Cells
  • Humans
  • Intestinal Mucosa / metabolism*
  • Intestines / cytology
  • Irritable Bowel Syndrome / diet therapy
  • Irritable Bowel Syndrome / metabolism
  • Lactobacillus acidophilus / metabolism*
  • Probiotics / metabolism
  • Probiotics / pharmacology
  • Serotonin Plasma Membrane Transport Proteins / genetics
  • Serotonin Plasma Membrane Transport Proteins / metabolism*
  • Up-Regulation*

Substances

  • SLC6A4 protein, human
  • Serotonin Plasma Membrane Transport Proteins